PRODUCT
Various applications
- Toxicology, Life span, Chemotaxis, Oxidative stress, Heat shock, etc.
Environmental control
- Equipped with temperature control and blue light control
Compatible with any culture media
- Can be used agar and liquid media
High-resolution and high-speed tracking
- High-resolution, high-density sensor tracks animal behavior at every step
FEATURES
It is able to measure behavior of any small animal cultured in microplates
- Measurement of animal activity in each well or group selected by the user
- Measurement time can be set up to 32000 minutes
- CSV file export and data analysis on original software
- Calcuration of average acticity, total activity, standard error and Map plot
The method is based on subjecting the organisms to an array of thousands of microbeams of light that are very close to each other. In this way, not only the movement of animals can be detected but also their position in the culture medium.
The innovation rely in applying a phenomenon called optical interferometry, which enables us to acquire shadows of small organisms without the need for microscopes or high resolution captures. It allows real-time processing.
This product is protected under international patents: P20060105084AR, PCT/IB2007/054628, EPO & US patent granted, owned by the Argentinian National Research Council CONICET and licensed to PHYLUMTECH S.A; and P20190100121AR, PCT/ES2020/070029, EPO & US patent owned by PHYLUMTECH S.A.
APPLICATION
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Levamisole Treatment In C. Elegans Cultured With NGM (ARENA System)
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Chemotaxis In C.Elegans (ARENA System)
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Heatshock Stress In C.Elegans (ARENA System)
Publication
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Developmental toxicity assessment of 4-MBC in Danio rerio embryo-larval stages
Ved Prakash, Veena Jain, Shweta Singh Chauhan, Ramakrishnan Parthasarathi, Somendu K. Roy, Sadasivam Anbumani
(System: ARENA, Model organism: Zebrafish)
PMID: 34509837 DOI: 10.1016/j.scitotenv.2021.149920
2022 Jan 15;804:149920. doi: 10.1016/j.scitotenv.2021.149920. Epub 2021 Sep 2.ABSTRACT:Enormous production of cosmetic products and its indiscriminate use tends to discharge into the aquatic environment and might threaten non-target organisms inhabiting aquatic ecosystems. In the present study, developmental toxicity of 4-methylbenzylidene camphor (4-MBC), a widely used organic UV filter in personal care
products has been evaluated using zebrafishembryo-larval stages.Waterborne exposure induced developmental toxicity and deduced 2.71 mg/L as 96 h LC50 whereas embryos exposed to sub-lethal concentrations (50 and 500 μg/L) caused a significant delay in hatching rate, heart rate, reduced larval length, and restricted hatchlings motility besides the axial curvature. Chronic exposure to 10 dpf resulted in significant decrease in SOD activity at 500 μg/L with no changes in CAT level besides a significant increase in GST enzyme at 5 μg/L concentration in 5 dpf sampled larvae. However, all the three enzymes were significantly elevated in 10 dpf larvae indicating differential oxidative stress during the stages of development. Similar trend is noticed for acetylcholine esterase enzymeactivity.A concentration dependent increase inmalondialdehyde contentwas noted in larvae sampled at 5 and 10 dpf. In addition, multixenobiotic resistance (MXR) activity inhibition, and elevated oxidative tissue damage were noticed at 5 dpf with no significant changes in 10 dpf larvae. Furthermore, immunoblot analysis confirms 4-MBC induced apoptosis in zebrafish larvae with promoted cleaved Caspase-3, Bax and inhibited
Bcl-2 proteins expression. Subsequently, docking studies revealed the binding potential of 4-MBC to zebrafishAbcb4 and CYP450 8A1 proteinswith the binding energy of−8.1 and−8.5 kcal/mol representing target proteins
SPECIFICATIONS
| WMicrotracker ARENA | |
| Microplate format compatibility | 6, 24 well pate, 35mm petri dish |
|---|---|
| Total IR channels | Sensing area composed of more than 20000 infrared microbeams |
| Organism Size | >= 1 mm in size |
| Picture acquisition | 1 frame/second |
| Data processing | 5 minutes time block resolution |
| Minimum measurement time (per plate) | 15-30min |
| Temperature control | Yes (20-37ºC) |
| Culture Media | Air, Agar, Liquid |
| Size | W260 × D205 × H270 mm |
Produst Name(code) |
Microplate format compatibility | Total IR channels | Organism Size | Picture acquisition | Data processing | Minimum measurement time (per plate) | Temperature control | Culture Media | Size |
|---|---|---|---|---|---|---|---|---|---|
| WMicrotracker ARENA | 6, 24 well pate, 35mm petri dish | Sensing area composed of more than 20000 infrared microbeams | >= 1 mm in size | 1 frame/second | 5 minutes time block resolution | 15-30min | Yes (20-37ºC) | Air, Agar, Liquid | W260 × D205 × H270 mm |