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Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC) -derived sensory neurons
Abstract
Chemotherapy-induced peripheral neuropathy (CIPN) is a frequent, potentially irreversible adverse effect of cytotoxic chemotherapy often leading to a reduction or discontinuation of treatment which negatively impacts patients’ prognosis. To date, however, neither predictive biomarkers nor preventive treatments for CIPN are available, which is partially due to a lack of suitable experimental models. We therefore aimed to evaluate whether sensory neurons derived from induced pluripotent stem cells (iPSC-DSN) can serve as human disease model system for CIPN. Treatment of iPSC-DSN for 24 h with the neurotoxic drugs paclitaxel, bortezomib, vincristine and cisplatin led to axonal blebbing and a dose dependent decline of cell viability in clinically relevant IC50 ranges, which was not observed for the non-neurotoxic compounds doxorubicin and 5-fluorouracil.
https://doi.org/10.1016/j.nbd.2021.105391 -
Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids
Junichi Takahashi Tomohiro Mizutani HadyYuki Sugihara Sei Kakinuma Mamoru Watanabe Ryuichi Okamoto
DOI:https://doi.org/10.1016/j.crmeth.2022.100337
Summary
Human intestinal organoids (HIOs) derived from human pluripotent stem cells (hPSCs) hold great promise for translational medical applications. A common method to obtain HIOs has been to harvest floating hindgut spheroids arising from hPSCs. As this technique is elegant but burdensome due to the complex protocol and line-to-line variability, a more feasible method is desired. Here, we establish a robust differentiation method into suspension-cultured HIOs (s-HIOs) by seeding dissociated cells on a spheroid-forming plate. This protocol realizes the reliable generation of size-controllable spheroids. Under optimized conditions in a rotating bioreactor, the generated spheroids quickly grow and mature into large s-HIOs with supporting mesenchyme. Upon mesenteric transplantation, s-HIOs further mature and develop complex tissue architecture in vivo. This method demonstrates that intestinal tissue can be generated from iPSC-derived HIOs via suspension induction and bioreactor maturation, establishing a reliable culture platform with wide applications in regenerative medicine. -
Derivation of pancreatic acinar cell carcinoma cell line HS-1 as a patient-derived tumor organoid
Daisuke Hoshi,Emiri Kita,Yoshiaki Maru,Hiroyuki Kogashi,Yuki Nakamura,Yasutoshi Tatsumi,Osamu Shimozato,Kazuyoshi Nakamura,Kentaro Sudo,Akiko Tsujimoto,Ryo Yokoyama,Atsushi Kato,Tetsuo Ushiku,Masashi Fukayama,Makiko Itami,Taketo Yamaguchi,Yoshitaka Hippo
https://doi.org/10.1111/cas.15656Abstract
Acinar cell carcinoma (ACC) of the pancreas is a malignant tumor of the exocrine cell lineage with a poor prognosis. Due to its rare incidence and technical difficulties, few authentic human cell lines are currently available, hampering detailed investigations of ACC. Therefore, we applied the organoid culture technique to various types of specimens, such as bile, biopsy, and resected tumor, obtained from a single ACC patient. Despite the initial propagation, none of these organoids achieved long-term proliferation or tolerated cryopreservation, confirming the challenging nature of establishing ACC cell lines. Nevertheless, the biopsy-derived early passage organoid developed subcutaneous tumors in immunodeficient mice. The xenograft tumor histologically resembled the original tumor and gave rise to infinitely propagating organoids with solid features and high levels of trypsin secretion.Moreover, the organoid stained positive for carboxylic ester hydrolase, a specific ACC marker, but negative for the duct cell marker CD133 and the endocrine lineage marker synaptophysin. Hence, we concluded the derivation of a novel ACC cell line of the pure exocrine lineage, designated HS-1. -
Fetal bovine serum enlarges the size of human pancreatic cancer spheres accompanied by an increase in the expression of cancer stem cell markers
Norihiko Sasaki, Masashi Toyoda, Fumio Hasegawa, Masakazu Fujiwara, Fujiya Gomi b, Toshiyuki Ishiwata.
Biochemical and Biophysical Research Communications, Vol. 514, Issue 1, 18 June 2019, Pages 112-117. -
Ganglioside GM2, highly expressed in the MIA PaCa-2 pancreatic ductal adenocarcinoma cell line, is correlated with growth, invasion, and advanced stage
Norihiko Sasaki, Kenichi Hirabayashi, Masaki Michishita, Kimimasa Takahashi, Fumio Hasegawa, Fujiya Gomi, Yoko Itakura, Naoya Nakamura, Masashi Toyoda,Toshiyuki Ishiwata
Scientific Reports volume 9, Article number: 19369 (2019) -
Hydrodynamic stress stimulates growth of cell clusters via the ANXA1/PI3K/AKT axis in colorectal cancer
Takeshi Hagihara, Jumpei Kondo, Hiroko Endo, Masayuki Ohue, Yoshiharu Sakai and Masahiro Inoue
Scientific Reports volume 9, Article number: 20027 (2019) -
Novel bone microenvironment model of prostate cancer with chitosan fiber matrix and osteoblast in 3D culture
Masahiro Samoto, Hiroaki Matsumoto, Hiroshi Hirata, Sho Ozawa, Junichi Mori, Ryo Inoue, Seiji Yano,
Yoshiaki Yamamoto and Hideyasu Matsuyama
AACR 10.1158/1538-7445.AM2020-321 Published August 2020 -
The anti-inflammatory effect of the gut lactic acid bacteria-generated metabolite 10-oxo-cis-6,trans-11-octadecadienoic acid on monocytes
Shuhei Hagiwara, Kazuki Nagata, Kazumi Kasakura, Fumiya Sakata, Shigenobu Kishino, Jun Ogawa, Takuya Yashiro and Chiharu Nishiyama
BBRC, Volume 530, Issue 1, 10 September 2020, Pages 342-347 -
Methods for Automated Single Cell Isolation and Sub‐Cloning of Human Pluripotent Stem Cells
Valeria Fernandez Vallone Narasimha Swamy Telugu Iris Fischer Duncan Miller Sandra Schommer Sebastian Diecke Harald Stachelscheid
Current Protocols in Stem Cell Biology,Volume55, Issue1,December 2020,e123 First published: 21 September 2020 -
Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media
Keisuke Sekine, Shimpei Ogawa, Syusaku Tsuzuki, Tatsuya Kobayashi, Kazuki Ikeda, Noriko Nakanishi, Kenta Takeuchi, Eriko Kanai, Yugo Otake, Satoshi Okamoto, Tsuyoshi Kobayashi, Takanori Takebe & Hideki Taniguchi
Scientific Reports volume 10, Article number: 17937 (2020) -
Functional analysis of isoflavones using patient-derived human colonic organoids
MaoTsuchiya, GoIto,MinamiHama, SayakaNagata, AmiKawamoto, KoheiSuzuki, HiromichiShimizu, ShoAnzai, JunichiTakahashi, ReikoKuno, SayakaTakeoka, YuiHiraguri, HadyYukiSugihara, TomohiroMizutani, ShiroYui, ShigeruOshima, KiichiroTsuchiya, MamoruWatanabe, RyuichiOkamoto
Biochemical and Biophysical Research Communications, Volume 542, 26 February 2021, Pages 40-47 -
Mammary cell gene expression atlas links epithelial cell remodeling events to breast carcinogenesis
Kohei Saeki, Gregory Chang, Noriko Kanaya, Xiwei Wu, Jinhui Wang, Lauren Bernal, Desiree Ha, Susan L. Neuhausen & Shiuan Chen
Communications Biology volume 4, Article number: 660 (2021)
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Polygenic architecture informs potential vulnerability to drug-induced liver injury
Masaru Koido, Eri Kawakami, Junko Fukumura, Yui Noguchi, Momoko Ohori, Yasunori Nio, Paola Nicoletti, Guruprasad P. Aithal, Ann K. Daly, Paul B. Watkins, Hisashi Anayama, Yvonne Dragan, Tadahiro Shinozawa & Takanori Takebe
Nature Medicine volume 26, pages1541–1548 (2020) Published: 07 September 2020 DOI:https://doi.org/10.1038/s41591-020-1023-0 -
Establishment and characterization of NCC-LMS2-C1—a novel patient-derived cancer cell line of leiomyosarcoma
Rei Noguchi, Yuki Yoshimatsu, Takuya Ono, Akane Sei, Kaoru Hirabayashi, Iwao Ozawa, Kazutaka Kikuta & Tadashi Kondo
Human Cell volume 34, pages279–288 (2021) Published: 01 October 2020 DOI:https://doi.org/10.1007/s13577-020-00443-6 -
Amphiregulin retains ERα expression in acquired aromatase inhibitor resistant breast cancer cells
Yuanzhong Wang, Yen-Dun Tony Tzeng, Gregory Chang, Xiaoqiang Wang, and Shiuan Chen
Endocrine-Related Cancer Volume/Issue: Volume 27: Issue 12 Page Range: 671–683 DOI: https://doi.org/10.1530/ERC-20-0258 online Publication Date: Dec 2020 -
Nickel ions attenuate autophagy flux and induce transglutaminase 2 (TG2) mediated post-translational modification of SQSTM1/p62
EmiAonuma ,AkikoTamura ,HirokiMatsuda ,TakehitoAsakawa ,YurikoSakamaki ,KanaOtsubo ,YoichiNibe ,MichioOnizawa ,YasuhiroNemoto ,TakashiNagaishi ,KiichiroTsuchiya ,TetsuyaNakamura ,MotohiroUo ,MamoruWatanabe ,RyuichiOkamoto ,ShigeruOshima
Biochemical and Biophysical Research Communications Volume 542, 26 February 2021, Pages 17-23 https://doi.org/10.1016/j.bbrc.2021.01.023 -
Functional analysis of isoflavones using patient-derived human colonic organoids
MaoTsuchiya ,GoIto ,MinamiHama ,SayakaNagata ,AmiKawamoto ,KoheiSuzuki ,HiromichiShimizu ,ShoAnzai ,JunichiTakahashi ,ReikoKuno ,SayakaTakeoka ,YuiHiraguri ,Hady YukiSugihara ,TomohiroMizutani ,ShiroYui ,ShigeruOshima ,KiichiroTsuchiya ,MamoruWatanabe ,RyuichiOkamoto
Biochemical and Biophysical Research Communications Volume 542, 26 February 2021, Pages 40-47 https://doi.org/10.1016/j.bbrc.2021.01.021 -
Polyvinyl alcohol increased growth, migration, invasion, and sphere size in the PK-8 pancreatic ductal adenocarcinoma cell line
Fujiya Gomi ,Norihiko Sasaki ,Yuuki Shichi ,Fuuka Minami ,Seiichi Shinji ,Masashi Toyoda ,Toshiyuki Ishiwata
Heliyon VOLUME 7, ISSUE 2, E06182, FEBRUARY 01, 2021DOI:https://doi.org/10.1016/j.heliyon.2021.e06182 -
Expansion and characterization of epithelial stem cells with potential for cyclical hair regeneration
Makoto Takeo, Kyosuke Asakawa, Koh-ei Toyoshima, Miho Ogawa, JingJing Tong, Tarou Irié, Masayuki Yanagisawa, Akio Sato & Takashi Tsuji
Scientific Reports volume 11, Article number: 1173 (2021) Published: 10 February 2021 -
Modeling chemotherapy induced neurotoxicity with human induced pluripotent stem cell (iPSC) -derived sensory neurons
ChristianSchinke ,ValeriaFernandez Vallone ,AndranikIvanov ,YangfanPeng ,PéterKörtvelyessy ,LucaNolte ,PetraHuehnchen ,DieterBeule ,HaraldStachelscheid ,WolfgangBoehmerle ,MatthiasEndres
Neurobiology of Disease Volume 155, July 2021, 105391 https://doi.org/10.1016/j.nbd.2021.105391 -
Lactate Reprograms Energy and Lipid Metabolism in Glucose-Deprived Oxidative Glioma Stem Cells
Noriaki Minami ORCID,Kazuhiro Tanaka ,Takashi Sasayama ,Eiji Kohmura ORCID,Hideyuki Saya andOltea Sampetrean
Metabolites 2021, 11(5), 325; https://doi.org/10.3390/metabo11050325 Published: 18 May 2021 -
Mammary cell gene expression atlas links epithelial cell remodeling events to breast carcinogenesis
Kohei Saeki, Gregory Chang, Noriko Kanaya, Xiwei Wu, Jinhui Wang, Lauren Bernal, Desiree Ha, Susan L. Neuhausen, and Shiuan Chen
Commun Biol. 2021; 4: 660. Published online 2021 Jun 2. doi:10.1038/s42003-021-02201-2 -
Novel bone microenvironment model of castration‑resistant prostate cancer with chitosan fiber matrix and osteoblasts
Masahiro Samoto Hideyasu Matsuyama Hiroaki Matsumoto Hiroshi Hirata Koji Ueno Sho Ozawa Junichi Mori Ryo Inoue Seiji Yano Yoshiaki Yamamoto Jun Haginaka Shizuyo Horiyama Koji Tamada
August 1, 2021 https://doi.org/10.3892/ol.2021.12950 Oncology Letters Article Number: 689