- APPLICATION
Cancer Research
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NK cell killing against 2D/3D-cultured cancer cells
In recent years, NK (Natural Killer) cells have been attracting attention in the fields of drug discovery development and cancer research. NK cells are a type of lymphocyte that works in innate immunity and play a major role in the removal of tumor cells and virus-infected cells.
By using Cell3iMager duos / duos2 and Deep Learning Plug-In, you will be able to perform a long-term Killing Assay. Since the imager uses a plate-fixed imaging method, clear imaging is possible even for suspended-cultured NK cells and 3D-cultured cancer cells. -
Real volume of spheroids
The volume of spheroids that stepwise cell number seeding, is quantified by using Cell3iMager Estier.
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Cell Viability and Cytotoxicity Assay
Viability of anti-cancer drug treated cells is quantified by using Cell3iMager duos.
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3D Cell Culture by using FCeM®
Multi spheroids cultured in FCeM-medium were quantified by using Cell3iMager duos.
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Assessing the growth of 3D spheroids cultured in Matrigel®
Visualisation and Quantification of spheroid volumes cultured in Cell3iMager duos.
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Confluency
The cell density that stepwise cell number seeding, is quantified by using Cell3iMager duos.
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Suspension cultured cells
Suspension cultured cells were quantified by using Cell3iMager duos.
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Real volume of spheroids
The volume of spheroids that stepwise cell number seeding, is quantified by using Cell3iMager Estier.
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Application note Vol.1
Development of spheroid-based anticancer drug screening method using high-speed cell culture scanner
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Application note Vol.3
High-Speed 3D Cell Scanner —Perfect for the Study of Complex 3D MCTS Models
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3D Invasion Assay
Invasiveness of MCF-7 (low malignant tumor) and MDA-MB-231 (high malignant tumor ) cells are compared by using Cell3iMager duos.
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Anti-Cancer Drug Screening
Anti-cancer drug screening in TCF4/LEF reporter (GFP) -expressing cells is performed by using Cell3iMager duos.
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Label free analysis of spheroids in microwells (Corning® Elplasia® Plates)
Spheroids in microwells of Corning®Elplasia® plate made by Corning are scanned and measured by Cell3iMager duos.
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3D cell culture by using hydrogels
Spheroids cultured in hydrogel were quantified by using Cell3iMager neo.
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3D Cell Culture in Gravity TRAP™
Spheroids formed by the method of hanging drop were moved to Gravity TRAPTM ULA plate, and quantified by Cell3iMager neo.
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Colony-forming units assay
Cell3iMager neo is useful for ‘colony formation assay’ stained by crystal violet
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3D Cell Culture by Hanging Drop
Spheroids in the droplets being aggregated by the method of hanging drop were quantified by Cell3iMager neo.