PRODUCT

High throughput

  • 96-well plates can be imaged in about 60 seconds and analyzed in about 30 seconds.
  • In addition, automatic capture of up to 200 plates per day is possible by connecting external devices such as plate stackers and incubators with a plate transfer robot.

3D cultured cells

  • 3D cultured cells can be analyzed with Z-stacking imaging and focus composition functions. Even cells scattered in the height direction can be captured clearly.

Deep Learning Cell Segmentation

  • Deep learning image processing enables "researcher's eye" segmentation of target cells. Accurate quantification is possible even for segmentation by cell morphology and high confluency, non-uniform images.

Multi-fluorescence imaging

  • Fluorescent color and bright field are automatically and continuously captured.
  • 5 types of fluorescent filters are available.

FEATURES

Meniscus-less

3D cellular imaging

Deep Learning Plug-in (optional)

Multi-fluorescence imaging

Automation system

Neurite elongation plug-in (optional)

Publication

  •  

    Novel bone microenvironment model of castration‑resistant prostate cancer with chitosan fiber matrix and osteoblasts

    Masahiro Samoto Hideyasu Matsuyama Hiroaki Matsumoto Hiroshi Hirata Koji Ueno Sho Ozawa Junichi Mori Ryo Inoue Seiji Yano Yoshiaki Yamamoto Jun Haginaka Shizuyo Horiyama Koji Tamada
    August 1, 2021 https://doi.org/10.3892/ol.2021.12950 Oncology Letters Article Number: 689

     
  •  

    Mammary cell gene expression atlas links epithelial cell remodeling events to breast carcinogenesis

    Kohei Saeki, Gregory Chang, Noriko Kanaya, Xiwei Wu, Jinhui Wang, Lauren Bernal, Desiree Ha, Susan L. Neuhausen, and Shiuan Chen
    Commun Biol. 2021; 4: 660. Published online 2021 Jun 2. doi:10.1038/s42003-021-02201-2

     
  •  

    Lactate Reprograms Energy and Lipid Metabolism in Glucose-Deprived Oxidative Glioma Stem Cells

    Noriaki Minami ORCID,Kazuhiro Tanaka ,Takashi Sasayama ,Eiji Kohmura ORCID,Hideyuki Saya andOltea Sampetrean
    Metabolites 2021, 11(5), 325; https://doi.org/10.3390/metabo11050325 Published: 18 May 2021

     

APPLICATION

Publication

  • Suspension culture in a rotating bioreactor for efficient generation of human intestinal organoids

    Junichi Takahashi Tomohiro Mizutani HadyYuki Sugihara Sei Kakinuma Mamoru Watanabe Ryuichi Okamoto
    DOI:https://doi.org/10.1016/j.crmeth.2022.100337
    Summary
    Human intestinal organoids (HIOs) derived from human pluripotent stem cells (hPSCs) hold great promise for translational medical applications. A common method to obtain HIOs has been to harvest floating hindgut spheroids arising from hPSCs. As this technique is elegant but burdensome due to the complex protocol and line-to-line variability, a more feasible method is desired. Here, we establish a robust differentiation method into suspension-cultured HIOs (s-HIOs) by seeding dissociated cells on a spheroid-forming plate. This protocol realizes the reliable generation of size-controllable spheroids. Under optimized conditions in a rotating bioreactor, the generated spheroids quickly grow and mature into large s-HIOs with supporting mesenchyme. Upon mesenteric transplantation, s-HIOs further mature and develop complex tissue architecture in vivo. This method demonstrates that intestinal tissue can be generated from iPSC-derived HIOs via suspension induction and bioreactor maturation, establishing a reliable culture platform with wide applications in regenerative medicine.

  • Derivation of pancreatic acinar cell carcinoma cell line HS-1 as a patient-derived tumor organoid

    Daisuke Hoshi,Emiri Kita,Yoshiaki Maru,Hiroyuki Kogashi,Yuki Nakamura,Yasutoshi Tatsumi,Osamu Shimozato,Kazuyoshi Nakamura,Kentaro Sudo,Akiko Tsujimoto,Ryo Yokoyama,Atsushi Kato,Tetsuo Ushiku,Masashi Fukayama,Makiko Itami,Taketo Yamaguchi,Yoshitaka Hippo
    https://doi.org/10.1111/cas.15656

    Abstract
    Acinar cell carcinoma (ACC) of the pancreas is a malignant tumor of the exocrine cell lineage with a poor prognosis. Due to its rare incidence and technical difficulties, few authentic human cell lines are currently available, hampering detailed investigations of ACC. Therefore, we applied the organoid culture technique to various types of specimens, such as bile, biopsy, and resected tumor, obtained from a single ACC patient. Despite the initial propagation, none of these organoids achieved long-term proliferation or tolerated cryopreservation, confirming the challenging nature of establishing ACC cell lines. Nevertheless, the biopsy-derived early passage organoid developed subcutaneous tumors in immunodeficient mice. The xenograft tumor histologically resembled the original tumor and gave rise to infinitely propagating organoids with solid features and high levels of trypsin secretion.Moreover, the organoid stained positive for carboxylic ester hydrolase, a specific ACC marker, but negative for the duct cell marker CD133 and the endocrine lineage marker synaptophysin. Hence, we concluded the derivation of a novel ACC cell line of the pure exocrine lineage, designated HS-1.

SPECIFICATIONS

Product name (Code) Cell3iMager duos2(CC-8300) Cell3iMager duos(CC-8000)
Channel Bright field and 3 Colors of Fluorescence Bright field and 1 Colors of Fluorescence
Bright field light source White LED strobes White LED strobes
Camera CMOS 4.2 megapixel color CMOS 4.2 megapixel color
Lenses Original hypercentric lens (High-speed mode)
Original telecentric lens (High-quality mode)
Original hypercentric lens (High-speed mode)
Original telecentric lens (High-quality mode)
Resolution 4.0μm/pixel (High-speed mode)
0.8μm/pixel (High-quality mode)
4.0μm/pixel (High-speed mode) /
0.8μm/pixel (High-quality mode)
Auto focus HW: Laser type real time auto focus
SW: Image contrast software auto focus
HW: Laser type real time auto focus
SW: Image contrast software auto focus
Image output 24bit color (8bit×3) 24bit color( 8bit×3)
Fluorescent filter type DAPI, GFP, Cy3, Texas Red, Cy5 -
Fluorescent light source (excitation wavelength) - U : 384nm , B : 470nm , G : 530nm , Y565nm , R : 625nm
Internal temperature 35℃±2℃ automatic adjustment, during the power is on 35℃±2℃ automatic adjustment, during the power is on (optional)
Placement environment temperature 18-28℃, humidity 80% or less, no condensation temperature 18-28℃, humidity 80% or less, no condensation
Transport conditions Packaged: 0-55℃, humidity 80% or less, no condensation Packaged: 0-55℃, humidity 80% or less, no condensation
Culture container 6・12・24・48・96・384 microwell plate (Compatible with almost all SBS standard plates)
35・60・100mm dish, slide glass (Optional adapter required)
6・12・24・48・96・384 microwell plate (Compatible with almost all SBS standard plates)
35・60・100mm dish, slide glass (Optional adapter required)
Power supply AC100-240V / 250VA AC100-240V / 250VA
Size and Weight W677xD580xH550 mm / 111 kg W677 x D570 x H550 mm / 110kg
Software Dedicated Cell3iMager software, includes as standard Dedicated Cell3iMager software, includes as standard
Designated computer with confirmed operation HP Z4 G4 workstation( SCREEN designated configuration), OS: Windows10 HP Z4 G4 workstation( SCREEN designated configuration), OS: Windows10
                                                                                                                      
Product name (Code) Channel Bright field light source Camera Lenses Resolution Auto focus Image output Fluorescent filter type Fluorescent light source (excitation wavelength) Internal temperature Placement environment Transport conditions Culture container Power supply Size and Weight Software Designated computer with confirmed operation
Cell3iMager duos2
(CC-8300)
Bright field and 3 Colors of FluorescenceWhite LED strobesCMOS 4.2 megapixel colorOriginal hypercentric lens (High-speed mode)
Original telecentric lens (High-quality mode)
4.0μm/pixel (High-speed mode)
0.8μm/pixel (High-quality mode)
HW: Laser type real time auto focus
SW: Image contrast software auto focus
24bit color (8bit×3)DAPI, GFP, Cy3, Texas Red, Cy5 -35℃±2℃ automatic adjustment, during the power is ontemperature 18-28℃, humidity 80% or less, no condensationPackaged: 0-55℃, humidity 80% or less, no condensation6・12・24・48・96・384 microwell plate (Compatible with almost all SBS standard plates)
35・60・100mm dish, slide glass (Optional adapter required)
AC100-240V / 250VAW677 x D580 x H550 mm / 111kgDedicated Cell3iMager software, includes as standardHP Z4 G4 workstation( SCREEN designated configuration), OS: Windows10
Cell3iMager duos
(CC-8000)
-U : 384nm , B : 470nm , G : 530nm ,
Y565nm , R : 625nm
35℃±2℃ automatic adjustment, during the power is on (optional)W677 x D570 x H550 mm / 110kg