PRODUCT

3D morphology and internal structure

  • OCT is the technique that can acquire the tomogram of the live cell or organism without aggression .Detailed observation for 3D structure of spheroids that conventional microscopes cannot recognize

Noninvasive and timelapse

  • Non-invasive, real-time observation of tomographic images and 3D morphology of spheroids, from their formation to collapse after addition of anti-cancer drugs, using OCT technology with weak near-infrared light

Easy operation

  • Simple operation on the PC screen.
  • Unnecessary of special training & expert technique.
  • Compatible with general micro well plates (SBS format) and culture dish.Because of the same workflow with microscope, it enables to reduce experiment cost and burden on researcher

FEATURES

3D morphological analyses for cultures / internal structures with OCT

OCT is the technique that can acquire the tomogram of the live cell or organism without aggression by using feeble near-infrared light.
Researchers can acquire 3D image safety and in real time without having any obstacles action for the living body as they use our device.

Observation of internal structure

Spheroid internal cavity morphology​(Prof. Tamio Mizukami, Nagahama Institute of Bio-Science and Technology)
Neovasculature​(Prof. Yukiko T. Matsunaga, University of Tokyo)

Tomographic imaging of living organisms/cell microstructure imaging

Ovary(Prof. Nobuo Nagai, Nagahama Institute of Bio-Science and Technology)
Kidney spheroid(Prof. Tetsuya Ohbayashi, Tottori University)

Time-lapse observation by non-invasive and label-free analysis

 

Compatibility for conventional labwares

Publication

  •  

    Development of a 3D Cell Culture System Using Amphiphilic Polydepsipeptides and Its Application to Hepatic Differentiation

    Junko Enomoto, Yukiko Toba, Haruka Yamazaki, Masaki Kanai, Hiroyuki Mizuguchi, and Hayato Matsui
    ACS Appl. Bio Mater. 2021, 4, 9, 7290–7299 Publication Date:September 8, 2021 https://doi.org/10.1021/acsabm.1c00816

     
  •  

    Development, Characterization and Potential Applications of a Multicellular Spheroidal Human Blood–Brain Barrier Model Integrating Three Conditionally Immortalized Cell Lines

    Keita Kitamura, Kenta Umehara, Ryo Ito, Yoshiyuki Yamaura, Takafumi Komori, Hanae Morio, Hidetaka Akita, Tomomi Furihata
    Biological and Pharmaceutical Bulletin 2021 Volume 44 Issue 7 Pages 984-991 DOI https://doi.org/10.1248/bpb.b21-00218

     
  •  

    Three-Dimensional Live Imaging of Bovine Preimplantation Embryos: A New Method for IVF Embryo Evaluation

    Yasumitsu Masuda, Ryo Hasebe, Yasushi Kuromi, Masayoshi Kobayashi, Kanako Urataki, Mitsugu Hishinuma, Tetsuya Ohbayashi and Ryo Nishimura
    Veterinary Reproductive Immunology Front. Vet. Sci., 26 April 2021 | https://doi.org/10.3389/fvets.2021.639249

     

APPLICATION

SPECIFICATIONS

Product name (Code) Cell3iMager Estier (CC-9000)
Data Tomogram in user indicated location / 3D image from user indicated view point / Movie output of tomogram / Animation output of 3D image
Output parameters Quantified value: distance between point to point, area of 2D image , volume, sphericity, surface rough degree, cavity volume
Light Source Near-infrared SLD light source (OCT type)
Max. FOV High resolution: 1 x 1 mm, Low resolution: 10 x 10 mm (Wide F.O.V.)
Max. depth 1,000um
Resolution High resolution: 3 um, Low resolution: 10 um
Cross-sectional observation time (e.g.) 0.5 sec. or more
3D observation time (e.g.) 3D observation: High resolution 0.3 x 0.3 x 0.3 mm / 3 μm: 1 min. / Low resolution 5.0 x 5.0 x 1.0 mm / 10 μm: 9 min.
Placement environment temperature 18-28℃, humidity 80% or less, no condensation
Transport conditions Packaged: 0-55℃, humidity 80% or less, no condensation
Culture container 6・12・24・48・96・384 microwell plate (Compatible with almost all SBS standard plates) / 35・60・100mm dish, slide glass (Optional adapter required)
Incubator Temperature (35~42°C), CO2 (Optional)
Power supply AC100-240V / 200VA
Size and Weight Main unit:W420 x D530 x H500 mm / 50kg Sub unit :W406 x D230 x H190 mm / 7kg
Software Dedicated Cell3iMager software, includes as standard
Designated computer with confirmed operation HP Z4 G4 workstation( SCREEN designated configuration), OS: Windows10
Product name (Code) Data Output parameters Light Source Max. FOV Max. depth Resolution Cross-sectional observation time (e.g.) 3D observation time (e.g.) Placement environment Transport conditions Culture container Incubator Power supply Size and Weight Software Designated computer
with confirmed operation
Cell3iMager Estier
(CC-9000)
Tomogram in user indicated location /
3D image from user indicated view point /
Movie output of tomogram /
Animation output of 3D image
Quantified value:
distance between point to point,
area of 2D image , volume, sphericity,
surface rough degree, cavity volume
Near-infrared SLD light source (OCT type) High resolution: 1 x 1 mm,
Low resolution: 10 x 10 mm
(Wide F.O.V.)
1,000um High resolution: 3 um,
Low resolution: 10 um
0.5 sec. or more 3D observation: High resolution 0.3 x 0.3 x 0.3 mm /
3 μm: 1 min. /
Low resolution 5.0 x 5.0 x 1.0 mm /
10 μm: 9 min.
temperature 18-28℃,
humidity 80% or less,
no condensation
Packaged: 0-55℃,
humidity 80% or less,
no condensation
6・12・24・48・96・384 microwell plate
(Compatible with almost
all SBS standard plates) / 35・60・100mm dish, slide glass
(Optional adapter required)
Temperature (35~42°C),
CO2 (Optional)
AC100-240V / 200VA Main unit:W420 x D530 x H500 mm /
50kg Sub unit :W406 x D230 x H190 mm /
7kg
Dedicated Cell3iMager software,
includes as standard
HP Z4 G4 workstation
(SCREEN designated configuration),
OS: Windows10